Regulatory
Ph-s

Part:BBa_K143015:Design

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-18)

Promoter hyper-spank for B. subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Biobrick standard was applied to the promoter hyper-spank sequence.


Source

The part was designed using the sequence from the B.subtilis genome and from previously published papers 123. This sequence was then synthesised by Geneart.


References

<biblio>

  1. 1 pmid=15028686
  2. 2 pmid=12169614
  3. 3 pmid=17720779

</biblio>